Mass Cytometry: Methods and Protocols. HM McGuire, TM Ashhurst (eds). 2019. Springer New York.
This book details a compilation of up-to-date and cutting-edge protocols in mass cytometry. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field.
‘Mass Cytometry: Methods and Protocols’ has been downloaded over 44,000 times. Protocols provided in this book have been cited in a wide variety of applications, including COVID-19 (Rodriguez et al. 2020, Koutsakos et al. 2021).
Newsletters etc - authored or featured
High Dimensional Data Analysis Strategies Homeshow 2020 - in ASI News. 2020. Australia and New Zealand Society for Immunology.
High-dimensional single cell mass cytometry (CyTOF) comes to Australia with the establishment of the Ramaciotti Facility for Human Systems Biology. 2015. T Ashhurst, N King, A Smith, BF de St Groth. ASI Newsletter.
Integrated immune dynamics define correlates of COVID-19 severity and antibody responses. 2021. M Koutsakos, LC Rowntree, L Hensen, … TM Ashhurst, … K Kedzierska. Cell Reports Medicine.
Intrapulmonary vaccination with delta-inulin adjuvant stimulates non-polarised chemotactic signalling and diverse cellular interaction. 2021. KC Ferrell, EL Stewart, C Counoupas, TM Ashhurst, WJ Britton, N Petrovsky, JA Triccas. Mucosal Immunology.
Using single-cell cytometry to illustrate integrated multi-perspective evaluation of clustering algorithms using Pareto fronts. 2021. GH Putri, I Koprinska, TM Ashhurst, NJC King, MN Read. Bioinformatics
- Code available from the Github repository.
Integration, exploration, and analysis of high-dimensional single-cell cytometry data using Spectre. 2020. TM Ashhurst*, F Marsh-Wakefield*, GH Putri*, AG Spiteri, D Shinko, … bioRxiv.
- Presentation at the Oz Single Cell webinar series: computational biology.
- Code available from our Github page
- Protocols and instructions available on the Spectre Home Page.
Evaluating spectral cytometry for immune profiling in viral disease. 2020. P Niewold*, TM Ashhurst*, AL Smith, NJC King. Cytometry Part A.
IgG3+ B cells are associated with the development of multiple sclerosis. 2020. F Marsh‐Wakefield, T Ashhurst, S Trend, HM McGuire, P Juillard, A Zinger, … Clinical & Translational Immunology 9 (5), e01133
Contribution of STAT1 to innate and adaptive immunity during type I interferon-mediated lethal virus infection. 2020. SR Jung, TM Ashhurst, PK West, B Viengkhou, NJC King, IL Campbell, … PLoS Pathogens 16 (4), e1008525
Zika virus encephalitis in immunocompetent mice is dominated by innate immune cells and does not require T or B cells. 2019. E Hayashida, ZL Ling, TM Ashhurst, B Viengkhou, SR Jung, … Journal of Neuroinflammation 16 (1), 1-15
Dimensionality Reduction for Clustering and Cluster Tracking of Cytometry Data. 2019. GH Putri, MN Read, I Koprinska, TM Ashhurst, NJC King. International Conference on Artificial Neural Networks, 624-640
ChronoClust: Density-based clustering and cluster tracking in high-dimensional time-series data. 2019. GH Putri, MN Read, I Koprinska, D Singh, U Röhm, TM Ashhurst, … Knowledge-Based Systems 174, 9-26
Cyt‐Geist: Current and future challenges in cytometry: Reports of the CYTO 2018 conference workshops. 2019. K Czechowska, J Lannigan, L Wang, J Arcidiacono, TM Ashhurst, … Cytometry Part A 95 (6), 598-644
Analysis of the murine bone marrow hematopoietic system using mass and flow cytometry. 2019. TM Ashhurst, DA Cox, AL Smith, NJC King. Mass Cytometry, 159-192
Staining of phosphorylated signalling markers protocol for mass cytometry. 2019. D Shinko, TM Ashhurst, HM McGuire, KA Charles. Mass Cytometry, 139-146
Collateral Damage: What Effect Does Anti-CD4 and Anti-CD8α Antibody–Mediated Depletion Have on Leukocyte Populations? 2018. SR Jung, T Suprunenko, TM Ashhurst, NJC King, MJ Hofer. The Journal of Immunology 201 (7), 2176-2186
Varicella zoster virus productively infects human natural killer cells and manipulates phenotype. 2018. TM Campbell, BP McSharry, M Steain, TM Ashhurst, B Slobedman, … PLoS pathogens 14 (4), e1006999
IRF9 Prevents CD8+ T cell exhaustion in an extrinsic manner during acute Lymphocytic choriomeningitis virus infection. 2017. M Huber, T Suprunenko, T Ashhurst, F Marbach, H Raifer, S Wolff, … Journal of Virology 91 (22)
High‐Dimensional Fluorescence Cytometry. 2017. TM Ashhurst, AL Smith, NJC King. Current Protocols in Immunology 119 (1), 5.8. 1-5.8. 38
Defective inflammatory monocyte development in IRF8-deficient mice abrogates migration to the West Nile virus-infected brain. 2015. RL Terry, C Deffrasnes, DR Getts, C Minten, C Van Vreden, TM Ashhurst, … Journal of innate immunity 7 (1), 102-112
The plasticity of inflammatory monocyte responses to the inflamed central nervous system. 2014. TM Ashhurst*, C van Vreden*, P Niewold*, NJC King. Cellular immunology 291 (1-2), 49-57
Therapeutic inflammatory monocyte modulation using immune-modifying microparticles. 2014. DR Getts, RL Terry, MT Getts, C Deffrasnes, M Müller, C van Vreden, … TM Ashhurst, … Science translational medicine 6 (219), 219ra7-219ra7.
Antiviral macrophage responses in flavivirus encephalitis. 2013. TM Ashhurst, C Van Vreden, L Munoz-Erazo, P Niewold, K Watabe, … The Indian journal of medical research 138 (5), 632
Targeted blockade in lethal West Nile virus encephalitis indicates a crucial role for very late antigen (VLA)-4-dependent recruitment of nitric oxide-producing macrophages. 2012. DR Getts, RL Terry, MT Getts, M Müller, S Rana, C Deffrasnes, … TM Ashhurst … Journal of Neuroinflammation 9 (1), 246