High-dimensional cytometry and single-cell technologies
Critical to investigating complex immune responses to disease is the capacity to measure cells at the single-cell level, examining multiple features of the cell simultaneously. To address this, we have developed and applied a variety of high-dimensional cytometry, single-cell, and spatial methods, through the Sydney Cytometry Core Research Facility. These technologies include high-dimensional flow cytometry (including the world’s first 10-laser 30-parameter ‘LSR-X’ platform developed with BD (Ashhurst et al. 2017), spectral cytometry, as well as Australia’s first mass cytometer (CyTOF) and Imaging Mass Cytometer (IMC). Most recently this has expanded to including single-cell transcriptomics and multi-omics.