King lab (2012 - 2016): inflammatory response of the bone marrow during viral encephalitis

Inflammatory response of the bone marrow during viral encephalitis

• BM mobilsation in viral encephalitis, starting with flow

Stanford University and Australia’s first CyTOF

• CyTOF (Stanford)
• CAPX
• Large flow panels
• Bone marrow mobilisation

High-dimensional flow cytometry

• PFC roadshow

Sydney Cytometry Facility (2017 - present): high-dimensional cytometry, spatial, and single-cell technologies

• HD flow with LSR-X – this with analysis strategies applied to range of projects (e.g. Hofer); and WNV/ZIKV
• Starting up IMC with the facility + analysis
• Spectral cytometry

• Now scRNAseq

• CYTO Asia State of the Art talk

• Numerous talks since

• CURRENTLY: HD spectral, IMC, analysis, scRNAseq, multiomics

Immune Dynamics initiative (2017 - present): development of novel single-cell and computational methods for single-cell systems immunology

• Started collaborative initiative between SC, path, and CS
• Initially with solving HD analysis and ‘time-series’ analysis problems
• MBI funding
• Spectre
• Timeseries: ChronoClust, TrackSOM
• Spatial analysis: SpectreMAP

• Human Cell atlas: Meetings in 2018, 2019 – HD cytometry and our approaches benefit from, and/or contribute to HCA

• Applied to COVID-19 and ZIKV, among others…

• Featured in OSC etc

• CURRENTLY: data integration an application to comprehensively map…