MASS CYTOMETRY (CyTOF): Below is a collection of resources and protocols for mass cytometry.
Our protocols:
- Paper: key considerations for generating high-quality data for analysis in high-dimensional cytometry (Marsh-Wakefield et al, 2021).
- Paper: Mass cytometry staining protocol for mouse bone marrow (Ashhurst et al 2019).
Protocols from Mass Cytometry: Methods and Protocols. HM McGuire, TM Ashhurst (eds). 2019. Springer New York:
Setting up a facility
- Setting Up Mass Cytometry in a Shared Resource Lab Environment (Sarah Warth, Désirée Kunkel): chapter.
- Acquisition, Processing, and Quality Control of Mass Cytometry Data (Brian H. Lee, Adeeb H. Rahman): chapter.
Panel Design and Reagent Preparation
- Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating (Amelia Au-Yeung, Chikara Takahashi, W. Rodney Mathews, William E. O’Gorman): chapter.
- Method for Tagging Antibodies with Metals for Mass Cytometry Experiments (Stephen Gregory Chang, Cynthia J. Guidos): chapter.
- Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis (Felix J. Hartmann, Erin F. Simonds, Nora Vivanco, Trevor Bruce, Luciene Borges, Garry P. Nolan et al.): chapter.
- Titration of Mass Cytometry Reagents (Caryn van Vreden, Paula Niewold, Helen M. McGuire, Barbara Fazekas de St. Groth): chapter.
- Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry (Axel Ronald Schulz, Henrik E. Mei): chapter.
Sample preparation
- Automated Cell Processing for Mass Cytometry Experiments (Jaromir Mikes, Axel Olin, Tadepally Lakshmikanth, Yang Chen, Petter Brodin): chapter.
- Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry (Lisa E. Wagar): chapter.
Specific applications
- Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry (Diana Shinko, Thomas M. Ashhurst, Helen M. McGuire, Kellie A. Charles): chapter.
- Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry (Yannick Simoni, Michael Fehlings, Evan W. Newell): chapter.
- Analysis of the Murine Bone Marrow Hematopoietic System Using Mass and Flow Cytometry (Thomas M. Ashhurst, Darren A. Cox, Adrian L. Smith, Nicholas J. C. King): chapter.
- Mass Cytometric Cell Cycle Analysis (Gregory K. Behbehani): chapter.
- Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry (Mikael Roussel, Todd Bartkowiak, Jonathan M. Irish): chapter.
- Quantitative Measurement of Cell-Nanoparticle Interactions Using Mass Cytometry (Andrew J. Mitchell, Angela Ivask, Yi Ju): chapter.
Data analysis
- Data-Driven Flow Cytometry Analysis (Sherrie Wang, Ryan R. Brinkman): chapter.
- Analysis of Mass Cytometry Data (Christina B. Pedersen, Lars R. Olsen): chapter.
- Analysis of High-Dimensional Phenotype Data Generated by Mass Cytometry or High-Dimensional Flow Cytometry (Branko Cirovic, Natalie Katzmarski, Andreas Schlitzer): chapter.
- Computational Analysis of High-Dimensional Mass Cytometry Data from Clinical Tissue Samples (Sam Norton, Roslyn Kemp): chapter.
- Supervised Machine Learning with CITRUS for Single Cell Biomarker Discovery (Hannah G. Polikowsky, Katherine A. Drake): chapter.